Sheep 5-hydroxytryptamine/serotonin(5HT/ST) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E14929Sh

Alternative Names

5-hydroxytryptamine/serotonin(5HT/ST)

Reactivity

Sheep

Detection Range

0.625 ng/mL-10 ng/mL

Sample Type

Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human sonic hedgehog spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-99
EDTA plasma (n=4)	88	82-94

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human sonic hedgehog in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-103
1:2	Average %	97
	Range %	92-105
1:4	Average %	98
	Range %	92-105
1:8	Average %	94
	Range %	88-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Ovis aries

Sensitivity

0.312 ng/mL

Limit Of Detection

450 nm

Antigen	5-hydroxytryptamine/serotonin(5HT/ST)

Applications

ELISA

Research Area

5HT, ST

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508854	CSB-E14929Sh	96T	$595	Add to Cart
BHE10508854	CSB-E14929Sh	5x96T	$2856	Add to Cart
BHE10508854	CSB-E14929Sh	10x96T	$5652.50	Add to Cart