This ELISA kit uses the Sandwich-Ag ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with recombinant SARS-CoV-2 Spike Protein. After adding samples (or controls) to wells, the total antibodies against SARS-CoV-2 Spike Protein will combine with the pre-coated SARS-CoV-2 Spike Protein. After washing completely, add Horseradish Peroxidase (HRP) conjugated SARS-CoV-2 Spike Protein to develop the antigen-antibody-HRP conjugated antigen complex. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SARS-CoV-2 Spike Protein, antibodies against SARS-CoV-2 Spike Protein and HRP conjugated SARS-CoV-2 Spike Protein will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. Compared with the CUT OFF value to judge whether SARS-CoV-2 Spike Protein total antibody exists in the tested samples or not.
Alternative Names
COVID 19 Spike Protein Total Antibody,Spike Protein Total Antibody,Spike Protein Antibody,SP Total Antibody,SP Antibody,SARS-CoV-2 Spike(RBD) Tab,2019-nCoV Spike(RBD) Tab,COVID-19 Spike(RBD) Tab
Detection Method
Colormetric
Recovery
80%-120%
Specificity
This kit recognizes SARS-CoV-2 Spike Protein Total Antibody in samples.No significant cross-reactivity or interference between Human SARS-CoV-2 Neutralization Antibody and analogues was observed
Precision
Intra-CV: CV%?8%; Inter-CV: CV%?10% Test Principle
Usage Notes
This ELISA kit applies to the in vitro Qualitative determination of SARS-CoV-2 Spike Protein Total Antibody in serum or plasma.
Applications
ELISA
Applications Notes
This ELISA kit applies to the in vitro Qualitative determination of SARS-CoV-2 Spike Protein Total Antibody in serum or plasma.
Research Area
SARS-CoV-2, COVID 19
Storage
An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is receIVed., 2-8°C/-20°C,12 months
Shipping
Ice bag
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