This ELISA kit uses Indirect-ELISA as the method to qualitatively detect the SARS-CoV-2 Nucleocapsid protein IgM in the sample. The micro ELISA plate provided in this kit is pre-coated with purified SARS-CoV-2 Nucleocapsid protein (E-ELC-001) antigen, after adding samples to wells, the SARS-CoV-2 Nucleocapsid protein IgM in the samples will combine with the pre-coated SARS-CoV-2 Nucleocapsid protein antigen. After washing completely, add Horseradish Peroxidase (HRP) conjugated mouse anti human IgM to develop the antigen-antibody-HRP conjugated secondary antibody complex. Free components are washed away, then the substrate solution is added to each well. Only those wells that contain SARS-CoV-2 Nucleocapsid protein IgM and HRP conjugated anti-human IgM will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. Compared with the CUT OFF value to judge whether SARS-CoV-2 Nucleocapsid protein IgM exists in the tested samples or not.
Alternative Names
COVID 19 IgM,N protein IgM
Detection Method
Colormetric
Recovery
80%-120%
Specificity
This kit recognizes Human SARS-CoV-2 IgM in samples.No significant cross-reactivity or interference between Human SARS-CoV-2 IgM and analogues was observed
Precision
Intra-CV: CV%?8%; Inter-CV: CV%?10% Test Principle
Usage Notes
This ELISA kit applies to the in vitro Qualitative determination of SARS-CoV-2 IgM in serum or plasma.
Applications
ELISA
Applications Notes
This ELISA kit applies to the in vitro Qualitative determination of SARS-CoV-2 IgM in serum or plasma.
Research Area
SARS-CoV-2, COVID 19
Storage
2-8°C,12 months
Shipping
Ice bag
Reviews of SARS-CoV-2 Nucleocapsid Protein IgM ELISA Kit