This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SARS-CoV-2 N protein. Samples (or Standards) and biotinylated detection antibody specific for SARS-CoV-2 N protein are added to the micro ELISA plate wells. SARS-CoV-2 N protein would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SARS-CoV-2 N protein, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of SARSCoV-2 N protein. You can calculate the concentration of SARS-CoV-2 N protein in the samples by comparing the OD of the samples to the standard curve.
Alternative Names
N protein, Protein N, NP, NC, Nucleoprotein, 2019-nCoV N protein
Detection Method
Colormetric
Recovery
80%-120%
Specificity
This kit recognizes SARS-CoV-2 N protein in samples.No significant cross-reactivity or interference between SARS-CoV-2 N protein and analogues was observed
Precision
Both intra-CV and inter-CV are < 10%. Test Principle
Usage Notes
This ELISA kit applies to the in vitro Quantitative determination of N protein in serum or plasma.
UniProt ID
P0DTC9
Applications
ELISA
Applications Notes
This ELISA kit applies to the in vitro Quantitative determination of N protein in serum or plasma.
Research Area
SARS-CoV-2, COVID 19
Storage
2-8°C/-20°C,12 months
Shipping
Ice bag
Reviews of SARS-CoV-2 Nucleocapsid Protein ELISA Kit