Matrices listed below were spiked with certain level of ZnT-3 and the recovery rates were calculated by comparing the measured value to the expected amount of ZnT-3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-101
96
EDTA plasma(n=5)
92-102
98
heparin plasma(n=5)
91-105
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ZnT-3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
93-102%
89-103%
92-105%
EDTA plasma(n=5)
86-97%
82-97%
87-100%
heparin plasma(n=5)
80-89%
80-100%
81-86%
Sensitivity
< 46.875pg/ml
Notes
For Research Use Only
UniProt ID
Q6QIX3
Applications
ELISA
Applications Notes
ZnT-3 ELISA Kit allows for the in vitro quantitative determination of ZnT-3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat ZnT-3(Zinc transporter 3) ELISA Kit