Matrices listed below were spiked with certain level of WNT5A and the recovery rates were calculated by comparing the measured value to the expected amount of WNT5A in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-100
91
EDTA plasma(n=5)
86-105
97
heparin plasma(n=5)
85-102
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of WNT5A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-105%
94-105%
87-104%
EDTA plasma(n=5)
88-97%
86-99%
84-99%
heparin plasma(n=5)
80-91%
80-99%
81-93%
Sensitivity
< 0.188ng/ml
Notes
For Research Use Only
UniProt ID
Q9QXQ7
Applications
ELISA
Applications Notes
WNT5A ELISA Kit allows for the in vitro quantitative determination of WNT5A concentrations in serum, plasma, tissue homogenates and other biological fluids.