Rat Vitamin D3 receptor(VDR) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL025832RA

Sensitivity

7.8 pg/mL

Detection Range

31.2 pg/mL-2000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

vitamin D (1,25- dihydroxyvitamin D3) receptor, NR1I1, vitamin D (1,25-dihydroxyvitamin D3) receptor

Recovery

The recovery of human Kim-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	96-108
EDTA plasma (n=4)	90	84-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human Kim-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	82-96
1:2	Average %	104
	Range %	99-109
1:4	Average %	100
	Range %	94-106
1:8	Average %	92
	Range %	85-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P13053

Applications	ELISA
Research Area	VDR

Storage	Store at 2-8°C. Please refer to protocol.

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