Rat Vimentin - VIM ELISA Kit

Mfr.No.

E2323Ra

Sensitivity

7.39ng/mL

Detection Range

15-3000ng/mL

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

VIM;Vimentin

Molecular Weight

53733

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2323Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	975.99	49.37	5.06
Intra-Assay	2	18	437.64	30.38	6.94
Intra-Assay	3	18	65.54	4.22	6.45

Background

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Protocol

These standard curves of E2323Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/mL	2.142	2.167	2.12
	2.191
800ng/mL	1.525	1.605	1.558
	1.685
400ng/mL	1.063	1.1	1.053
	1.137
200ng/mL	0.752	0.761	0.715
	0.771
100ng/mL	0.469	0.461	0.414
	0.453
0ng/mL	0.03	0.047	0
	0.063

Gene ID	81818
UniProt ID	P31000

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113455	E2323Ra	48T	$320		Add to Cart
BHE12113455	E2323Ra	96T	$458		Add to Cart

Rat Vimentin - VIM ELISA Kit

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