Rat very low density lipoprotein(VLDL)ELISA Kit

Mfr.No.

CSB-E17088r

Alternative Names

very low density lipoprotein(VLDL)

Reactivity

Rat

Detection Range

3.12 ng/mL-200 ng/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of mouse SERPINE2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SERPINE2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	103
	Range %	99-112
1:200	Average %	84
	Range %	80-88
1:400	Average %	97
	Range %	92-101
1:800	Average %	90
	Range %	85-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.185	2.312	2.249	2.111
100	1.841	1.937	1.889	1.751
50	1.437	1.385	1.411	1.273
25	0.946	1.002	0.974	0.836
12.5	0.598	0.579	0.589	0.451
6.25	0.362	0.374	0.368	0.230
3.12	0.235	0.226	0.231	0.093
0	0.134	0.141	0.138	?

Species

Rattus norvegicus

Sensitivity

0.78 ng/mL

Limit Of Detection

450 nm

Antigen	very low density lipoprotein(VLDL)

Applications

ELISA

Research Area

VLDL

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Morin reduces hepatic inflammation-associated lipid accumulation in high fructose-fed rats via inhibiting sphingosine kinase 1/sphingosine 1-phosphate signaling pathway Wang X et al,Biochem Pharmacol,2013 Oct 14.

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BHE10508817	CSB-E17088r	96T	$595	Add to Cart
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BHE10508817	CSB-E17088r	10x96T	$5652.50	Add to Cart

Rat very low density lipoprotein(VLDL)ELISA Kit

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