Rat very low density lipoprotein(VLDL)ELISA Kit

Mfr.No.

CSB-E17088r

Sensitivity

0.78 ng/mL

Detection Range

3.12 ng/mL-200 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

very low density lipoprotein(VLDL)

Recovery

The recovery of mouse SERPINE2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SERPINE2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	103
	Range %	99-112
1:200	Average %	84
	Range %	80-88
1:400	Average %	97
	Range %	92-101
1:800	Average %	90
	Range %	85-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	VLDL

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Morin reduces hepatic inflammation-associated lipid accumulation in high fructose-fed rats via inhibiting sphingosine kinase 1/sphingosine 1-phosphate signaling pathway Wang X et al,Biochem Pharmacol,2013 Oct 14.

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