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Home / ELISA Kit

Rat Very low-density lipoprotein receptor (VLDLR) ELISA Kit

SKU : BHE13700156

Sugie et al. (2005) classified XMEA as a form of autophagic vacuolar myopathy, characterized by intracytoplasmic autophagic vacuoles with sarcolemmal features.The clinical course was mild; the patients suffered from slowly progressive muscle weakness mainly in the legs, but did not lose their ability to walk. There was no evidence of cardiac or neural involvement. Serum creatine kinase was elevated. By electron microscopy, an excessive number of autophagic vacuoles with staining properties of lysosomes were observed. The granular and membranous material contained in these vacuoles was actively exocytosed. The authors suggested that this disorder differed from the muscular dystrophy of Duchenne and Becker and of Emery-Dreifuss as well as from X-linked myotubular myopathy.

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Mfr.No.	KTE100007
Reactivity	Rat
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Rat Very low-density lipoprotein receptor (VLDLR) ELISA Kit employs a two-site sandwich ELISA to quantitate VLDLR.
Alternative Names	VLDLR; RP11-320E16.1; CHRMQ1; FLJ35024; VLDLRCH;
Detection Method	Colorimetric
Kit Components	Rat Very low-density lipoprotein receptor microplate Rat Very low-density lipoprotein receptor standard Rat Very low-density lipoprotein receptor detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Rat Very low-density lipoprotein receptor (VLDLR) ELISA Kit has high sensitivity and excellent specificity for detection of Rat VLDLR. No significant cross-reactivity or interference between Rat VLDLR and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Sugie et al. (2005) classified XMEA as a form of autophagic vacuolar myopathy, characterized by intracytoplasmic autophagic vacuoles with sarcolemmal features.The clinical course was mild; the patients suffered from slowly progressive muscle weakness mainly in the legs, but did not lose their ability to walk. There was no evidence of cardiac or neural involvement. Serum creatine kinase was elevated. By electron microscopy, an excessive number of autophagic vacuoles with staining properties of lysosomes were observed. The granular and membranous material contained in these vacuoles was actively exocytosed. The authors suggested that this disorder differed from the muscular dystrophy of Duchenne and Becker and of Emery-Dreifuss as well as from X-linked myotubular myopathy.
Notes	This Rat Very low-density lipoprotein receptor (VLDLR) ELISA Kit employs a two-site sandwich ELISA to quantitate VLDLR in samples. An antibody specific for VLDLR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVLDLR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VLDLR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VLDLR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	100008976

Applications	ELISA
Applications Notes	This Rat Very low-density lipoprotein receptor (VLDLR) ELISA Kit employs a two-site sandwich ELISA to quantitate VLDLR in samples. An antibody specific for VLDLR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVLDLR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VLDLR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VLDLR bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13700156	KTE100007	48 T	$339		Add to Cart
BHE13700156	KTE100007	96 T	$589		Add to Cart
BHE13700156	KTE100007	96 Tx5	$2629		Add to Cart
BHE13700156	KTE100007	96 Tx50	$24569		Add to Cart

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