Rat Vasoactive Intestinal Peptide

Mfr.No.

E0652Ra

Sensitivity

0.27ng/L

Detection Range

0.5-200ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Vasoactive Intestinal Peptide (also known as VIP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Intestinal Peptide PHI-27;Peptide histidine isoleucinamide 27 ;Vasoactive intestinal Peptide;Vasoactive intestinal polypeptide;VIP;VIP Peptides Intestinal Peptide PHV-42

Molecular Weight

19079

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0652Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	7.6	0.43	5.7
Intra-Assay	2	18	41.8	1.22	2.9
Intra-Assay	3	18	29.1	2.1	7.2

Background

VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder. PHM-27 is a potent agonist of the calcitonin receptor CALCR, with similar efficacy as calcitonin (By similarity). PHI also causes vasodilation.

Protocol

These standard curves of E0652Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/L	2.324	2.316	2.263
	2.308
60ng/L	1.713	1.699	1.647
	1.686
30ng/L	1.304	1.308	1.256
	1.313
15ng/L	0.605	0.65	0.597
	0.696
7.5ng/L	0.425	0.421	0.368
	0.418
0ng/L	0.045	0.053	0
	0.06

Gene ID	117064
UniProt ID	P01283

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111921	E0652Ra	48T	$320		Add to Cart
BHE12111921	E0652Ra	96T	$458		Add to Cart

Rat Vasoactive Intestinal Peptide - VIP ELISA Kit

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