Rat Vascular Endothelial cell Growth Factor D,VEGF-D ELISA kit

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E07352r

Alternative Names

c-fos induced growth factor (vascular endothelial growth factor D), VEGF-D, VEGFD, vascular endothelial growth factor D

Reactivity

Rat

Detection Range

0.312 ng/mL-20 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of fish TNF-? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-102
EDTA plasma (n=4)	95	91-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of fish TNF-? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-100
1:2	Average %	93
	Range %	87-99
1:4	Average %	95
	Range %	88-103
1:8	Average %	96
	Range %	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Sensitivity

0.078 ng/mL

Limit Of Detection

450 nm

UniProt ID	O35251
Antigen	c-fos induced growth factor (vascular endothelial growth factor D)

Applications

ELISA

Research Area

Vegfd

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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