Rat Vascular Cell Adhesion Protein 1

Mfr.No.

E2318Ra

Sensitivity

3.04ng/mL

Detection Range

6-1800ng/mL

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

CD antigen CD106;Vascular cell adhesion protein 1;V-CAM 1;VCAM-1;VCAM1B

Molecular Weight

81246

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2318Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	336.52	16.15	4.8
Intra-Assay	2	18	748.22	27.21	3.64
Intra-Assay	3	18	121.03	7.5	6.2

Background

Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.

Protocol

These standard curves of E2318Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
960ng/mL	2.185	2.129	2.076
	2.072
480ng/mL	1.231	1.252	1.2
	1.273
240ng/mL	0.726	0.751	0.699
	0.776
120ng/mL	0.437	0.416	0.363
	0.394
60ng/mL	0.181	0.178	0.125
	0.174
0ng/mL	0.044	0.052	0
	0.061

Gene ID	25361
UniProt ID	P29534

Research Area	Signal Transduction; Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113450	E2318Ra	48T	$320		Add to Cart
BHE12113450	E2318Ra	96T	$458		Add to Cart

Rat Vascular Cell Adhesion Protein 1 - VCAM-1 ELISA Kit

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