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Home / ELISA Kit

Rat Urotensin-2 (UTS2) ELISA Kit

SKU : BHE13700172

A novel peptide, UII-related peptide (URP), from the extract of the rat brain as the sole immunoreactive substance to anti-UII antibody. cDNAs encoding rat, mouse, and human precursor proteins for URP were cloned and revealed that the sequences of mouse and human URP peptides are the same as that for rat URP. Prepro-URP gene is expressed in several rat tissues such as those of the thymus, spleen, testis, and spinal cord, although with lower levels than the prepro-UII gene. In the human, the prepro-URP gene is expressed comparably to prepro-UII in several tissues except the spinal cord. URP was found to bind and activate the human or rat UII receptors (GPR14) and showed a hypotensive effect when administered to anesthetized rats. URP is the endogenous and functional ligand for UII receptor in the rat and mouse, and possibly in the human.

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Mfr.No.	KTE100022
Reactivity	Rat
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Rat Urotensin-2 (UTS2) ELISA Kit employs a two-site sandwich ELISA to quantitate UTS2.
Alternative Names	UTS2; PRO1068; U-II; UCN2; UII;
Detection Method	Colorimetric
Kit Components	Rat Urotensin-2 microplate Rat Urotensin-2 standard Rat Urotensin-2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Rat Urotensin-2 (UTS2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat UTS2. No significant cross-reactivity or interference between Rat UTS2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	A novel peptide, UII-related peptide (URP), from the extract of the rat brain as the sole immunoreactive substance to anti-UII antibody. cDNAs encoding rat, mouse, and human precursor proteins for URP were cloned and revealed that the sequences of mouse and human URP peptides are the same as that for rat URP. Prepro-URP gene is expressed in several rat tissues such as those of the thymus, spleen, testis, and spinal cord, although with lower levels than the prepro-UII gene. In the human, the prepro-URP gene is expressed comparably to prepro-UII in several tissues except the spinal cord. URP was found to bind and activate the human or rat UII receptors (GPR14) and showed a hypotensive effect when administered to anesthetized rats. URP is the endogenous and functional ligand for UII receptor in the rat and mouse, and possibly in the human.
Notes	This Rat Urotensin-2 (UTS2) ELISA Kit employs a two-site sandwich ELISA to quantitate UTS2 in samples. An antibody specific for UTS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUTS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UTS2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UTS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	29180

Applications	ELISA
Applications Notes	This Rat Urotensin-2 (UTS2) ELISA Kit employs a two-site sandwich ELISA to quantitate UTS2 in samples. An antibody specific for UTS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUTS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UTS2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UTS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13700172	KTE100022	48 T	$339		Add to Cart
BHE13700172	KTE100022	96 T	$589		Add to Cart
BHE13700172	KTE100022	96 Tx5	$2629		Add to Cart
BHE13700172	KTE100022	96 Tx50	$24569		Add to Cart

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