Rat UDP-glucumno-syltransferase 1(UGT1) ELISA Kit

Mfr.No.

CSB-E13845r

Sensitivity

7.8 pg/mL

Detection Range

31.2 pg/mL-2000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

UDP glucuronosyltransferase 1 family, polypeptide A1, GNT1, HUG-BR1, UDPGT, UGT1, UGT1A, UDP glucuronosyltransferase 1A1, UDP glycosyltransferase 1 family, polypeptide A1, bilirubin UDP-glucuronosyltransferase 1-1, bilirubin UDP-glucuronosyltransferase is

Recovery

The recovery of rat CD4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-98
EDTA plasma (n=4)	98	89-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat CD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	97
	Range %	87-102
1:2	Average %	101
	Range %	94-105
1:4	Average %	99
	Range %	88-103
1:8	Average %	105
	Range %	98-109

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

U/ml	OD1	OD2	Average	Corrected
50	2.656	2.587	2.622	2.508
25	2.171	2.062	2.117	2.003
12.5	1.438	1.388	1.413	1.299
6.25	0.720	0.715	0.718	0.604
3.12	0.412	0.424	0.418	0.304
1.56	0.261	0.252	0.257	0.143
0.78	0.176	0.183	0.180	0.066
0	0.112	0.115	0.114	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q64550

Applications	ELISA
Research Area	UGT1A1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508794	CSB-E13845r	96T	$790	Add to Cart
BHE10508794	CSB-E13845r	5x96T	$3792	Add to Cart
BHE10508794	CSB-E13845r	10x96T	$7505	Add to Cart

Rat UDP-glucumno-syltransferase 1(UGT1) ELISA Kit

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