Matrices listed below were spiked with certain level of UBL4A and the recovery rates were calculated by comparing the measured value to the expected amount of UBL4A in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-102
95
EDTA plasma(n=5)
90-103
96
heparin plasma(n=5)
88-96
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of UBL4A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-96%
86-104%
90-103%
EDTA plasma(n=5)
83-98%
85-98%
82-98%
heparin plasma(n=5)
80-94%
87-100%
83-96%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
B2GV38
Applications
ELISA
Applications Notes
UBL4A ELISA Kit allows for the in vitro quantitative determination of UBL4A concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat UBL4A (Ubiquitin-like protein 4A) ELISA Kit