Rat Ubiquitin carboxyl-terminal hydrolase isozyme L1(UCHL1) ELISA kit

Rat Ubiquitin carboxyl-terminal hydrolase isozyme L1(UCHL1) ELISA kit

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Mfr.No.

CSB-EL025541RA

Alternative Names

ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase), PARK5, PGP9.5, PGP95, Uch-L1, neuron cytoplasmic protein 9.5, ubiquitin C-terminal hydrolase, ubiquitin carboxyl-terminal esterase L1

Reactivity

Rat

Detection Range

0.78 ng/mL-50 ng/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse HSP90AB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	84-105
EDTA plasma (n=4)	97	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse HSP90AB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	85-98
1:2	Average %	89
	Range %	81-100
1:4	Average %	96
	Range %	92-105
1:8	Average %	91
	Range %	85-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.014	2.132	2.073	1.898
500	1.378	1.355	1.367	1.192
250	0.748	0.726	0.737	0.562
125	0.432	0.422	0.427	0.252
62.5	0.299	0.309	0.304	0.129
31.25	0.245	0.253	0.249	0.074
15.6	0.217	0.227	0.222	0.047
0	0.173	0.176	0.175

Species

Rattus norvegicus

Sensitivity

0.195 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q00981
Antigen	ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)

Applications

ELISA

Research Area

UCHL1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508791	CSB-EL025541RA	96T	$695	Add to Cart
BHE10508791	CSB-EL025541RA	5x96T	$3336	Add to Cart
BHE10508791	CSB-EL025541RA	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.