Rat tumor necrosis factor-related apoptosis inducing ligand,TRAIL ELISA Kit

Mfr.No.

CSB-E13904r

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

tumor necrosis factor-related apoptosis inducing ligand,TRAIL

Recovery

The recovery of mouse CD248 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-102
EDTA plasma (n=4)	98	94-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CD248 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	95
	Range %	90-99
1:200	Average %	102
	Range %	96-107
1:400	Average %	93
	Range %	89-98
1:800	Average %	86
	Range %	80-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P50591

Applications	ELISA
Research Area	TRAIL

Storage	Store at 2-8°C. Please refer to protocol.

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