Rat Tumor necrosis factor ligand superfamily member 15(TNFSF15) ELISA kit

Rat Tumor necrosis factor ligand superfamily member 15(TNFSF15) ELISA kit

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Mfr.No.

CSB-EL023992RA

Sensitivity

6.25 pg/mL

Detection Range

25 pg/mL-1600 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

tumor necrosis factor (ligand) superfamily, member 15, MGC129934, MGC129935, TL1, TL1A, VEGI, VEGI192A, TNF ligand-related molecule 1, TNF superfamily ligand TL1A, vascular endothelial cell growth inhibitor, vascular endothelial growth inhibitor-192A

Recovery

The recovery of human sortilin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-97
EDTA plasma (n=4)	97	93-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human sortilin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-93
1:2	Average %	104
	Range %	100-107
1:4	Average %	94
	Range %	88-97
1:8	Average %	99
	Range %	93-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
3000	2.349	2.333	2.341	2.184
1500	1.864	1.776	1.820	1.663
750	1.312	1.302	1.307	1.150
375	0.943	0.922	0.933	0.776
187.5	0.654	0.634	0.644	0.487
93.75	0.463	0.456	0.460	0.303
46.88	0.317	0.304	0.311	0.154
0	0.158	0.156	0.157	?

Species

Rattus norvegicus

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q8K3Y7

Applications	ELISA
Research Area	TNFSF15

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508778	CSB-EL023992RA	96T	$595	Add to Cart
BHE10508778	CSB-EL023992RA	5x96T	$2856	Add to Cart
BHE10508778	CSB-EL023992RA	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.