Rat tumor necrosis factor (ligand) superfamily, member 14 (TNFSF14) ELISA kit

Mfr.No.

CSB-EL023991RA

Alternative Names

tumor necrosis factor (ligand) superfamily, member 14 (TNFSF14)

Reactivity

Rat

Detection Range

15.6 pg/mL-1000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human SGK1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-103
EDTA plasma (n=4)	94	89-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SGK1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	91
	Range %	85-96
1:2	Average %	97
	Range %	91-103
1:4	Average %	102
	Range %	97-108
1:8	Average %	92
	Range %	86-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Sensitivity

3.9 pg/mL

Limit Of Detection

450 nm

Antigen	tumor necrosis factor (ligand) superfamily, member 14 (TNFSF14)

Applications

ELISA

Research Area

TNFSF14

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10508777	CSB-EL023991RA	96T	$595	Add to Cart
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