Rat tumor necrosis factor-inducible gene 6 protein(TSG-6) elisa kit

Rat tumor necrosis factor-inducible gene 6 protein(TSG-6) elisa kit

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Mfr.No.

CSB-E17373r

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

tumor necrosis factor-inducible gene 6 protein(TSG-6), TSG-6, TSG6, hyaluronate-binding protein, tumor necrosis factor alpha-inducible protein 6, tumor necrosis factor-inducible protein 6, tumor necrosis factor-stimulated gene-6 protein

Recovery

The recovery of human GP-? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	97	93-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GP-? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-94
1:2	Average %	104
	Range %	98-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
160	2.783	2.777	2.780	2.663
80	2.134	2.117	2.126	2.009
40	1.496	1.478	1.487	1.370
20	0.855	0.837	0.846	0.729
10	0.417	0.404	0.411	0.294
5	0.321	0.334	0.328	0.211
2.5	0.225	0.216	0.221	0.104
0	0.118	0.116	0.117	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	TSG-6

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508776	CSB-E17373r	96T	$790	Add to Cart
BHE10508776	CSB-E17373r	5x96T	$3792	Add to Cart
BHE10508776	CSB-E17373r	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.