Matrices listed below were spiked with certain level of Ttr and the recovery rates were calculated by comparing the measured value to the expected amount of Ttr in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-104
95
EDTA plasma(n=5)
89-103
96
heparin plasma(n=5)
89-95
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ttr and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-101%
86-103%
92-105%
EDTA plasma(n=5)
93-101%
90-101%
90-99%
heparin plasma(n=5)
91-100%
91-100%
90-98%
Sample Type
Other liquid samples, Serum, Cell culture supeatants, Cell lysate, Plasma
Ttr/PALB/TBPA/ATTR/Prealbumin/HsT2651/prealbumin, amyloidosis type I/thyroxine-binding prealbumin
Notes
For Research Use Only
UniProt ID
P02767
Applications
ELISA
Applications Notes
Ttr ELISA Kit allows for the in vitro quantitative determination of Ttr concentrations in serum, plasma, tissue homogenates and other biological fluids.