Matrices listed below were spiked with certain level of TSP-1 and the recovery rates were calculated by comparing the measured value to the expected amount of TSP-1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-102
96
EDTA plasma(n=5)
85-100
93
heparin plasma(n=5)
98-100
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TSP-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
TSP-1 ELISA Kit allows for the in vitro quantitative determination of TSP-1 concentrations in serum, plasma, tissue homogenates and other biological fluids.