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Home / ELISA Kit

Rat Tryptophan Hydroxylase (TPH) ELISA Kit

SKU : BHE13700194

TPH2 encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. The human genome contains two related tryptophan hydroxylases, one on chromosome 11p15-p14 and one on chromosome 12q21. This gene is expressed predominantly in the brain stem. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression.
This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by the mouse ortholog.

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Mfr.No.	KTE100042
Reactivity	Rat
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Rat Tryptophan Hydroxylase (TPH) ELISA Kit employs a two-site sandwich ELISA to quantitate TPH1.
Alternative Names	TPH1; MGC119994; TPRH; TRPH; L-tryptophan hydroxylase; indoleacetic acid-5-hydroxylase; tryptophan hydroxylase (tryptophan 5-monooxygenase)
Detection Method	Colorimetric
Kit Components	Rat Tryptophan Hydroxylase microplate Rat Tryptophan Hydroxylase standard Rat Tryptophan Hydroxylase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Rat Tryptophan Hydroxylase (TPH) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TPH1. No significant cross-reactivity or interference between Rat TPH1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TPH2 encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. The human genome contains two related tryptophan hydroxylases, one on chromosome 11p15-p14 and one on chromosome 12q21. This gene is expressed predominantly in the brain stem. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression. This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by the mouse ortholog.
Notes	This Rat Tryptophan Hydroxylase (TPH) ELISA Kit employs a two-site sandwich ELISA to quantitate TPH1 in samples. An antibody specific for TPH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTPH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	100009100

Applications	ELISA
Applications Notes	This Rat Tryptophan Hydroxylase (TPH) ELISA Kit employs a two-site sandwich ELISA to quantitate TPH1 in samples. An antibody specific for TPH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTPH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13700194	KTE100042	48 T	$359		Add to Cart
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BHE13700194	KTE100042	96 Tx50	$26209		Add to Cart

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