Rat Tryptophan Hydroxylase (TPH) ELISA Kit

Rat Tryptophan Hydroxylase (TPH) ELISA Kit

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Mfr.No.

CSB-E13984r

Alternative Names

tryptophan hydroxylase 1, MGC119994, TPRH, TRPH, L-tryptophan hydroxylase, indoleacetic acid-5-hydroxylase, tryptophan hydroxylase (tryptophan 5-monooxygenase)

Reactivity

Rat

Detection Range

37.5 pg/mL-2400 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human CD177 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-100
EDTA plasma (n=4)	94	89-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CD177 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	103
	Range %	95-108
1:2	Average %	86
	Range %	80-92
1:4	Average %	92
	Range %	87-96
1:8	Average %	96
	Range %	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
60	2.635	2.863	2.749	2.611
30	1.835	1.995	1.915	1.777
15	1.158	1.256	1.207	1.069
7.5	0.550	0.533	0.542	0.404
3.75	0.321	0.341	0.331	0.193
1.8	0.251	0.249	0.250	0.112
0.9	0.200	0.206	0.203	0.065
0	0.139	0.137	0.138	?

Species

Rattus norvegicus

Sensitivity

9.4 pg/mL

Limit Of Detection

450 nm

UniProt ID	P09810
Antigen	Tryptophan Hydroxylase 1

Applications

ELISA

Research Area

TPH1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508775	CSB-E13984r	96T	$790	Add to Cart
BHE10508775	CSB-E13984r	5x96T	$3792	Add to Cart
BHE10508775	CSB-E13984r	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.