Matrices listed below were spiked with certain level of TrxR1 and the recovery rates were calculated by comparing the measured value to the expected amount of TrxR1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-100
92
EDTA plasma(n=5)
90-105
97
heparin plasma(n=5)
90-104
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TrxR1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
TrxR1 ELISA Kit allows for the in vitro quantitative determination of TrxR1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat TrxR1(Thioredoxin Reductase 1) ELISA Kit