Rat Troponin I, slow skeletal muscle(TNNI1) ELISA kit

Rat Troponin I, slow skeletal muscle(TNNI1) ELISA kit

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Mfr.No.

CSB-EL024011RA

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

troponin I type 1 (skeletal, slow), DKFZp451O223, SSTNI, TNN1, OTTHUMP00000033877, OTTHUMP00000033910, slow-twitch skeletal troponin I, troponin I, skeletal, slow

Recovery

The recovery of human SOST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-99
EDTA plasma (n=4)	90	85-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SOST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-92
1:2	Average %	95
	Range %	90-100
1:4	Average %	96
	Range %	90-101
1:8	Average %	93
	Range %	88-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	1.932	1.904	1.918	1.808
1000	1.516	1.481	1.499	1.389
500	1.112	1.101	1.107	0.997
250	0.733	0.687	0.710	0.600
125	0.547	0.504	0.526	0.416
62.5	0.329	0.309	0.319	0.209
31.25	0.197	0.181	0.189	0.079
0	0.112	0.107	0.110	?

Species

Rattus norvegicus

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P13413

Applications	ELISA
Research Area	TNNI1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508768	CSB-EL024011RA	96T	$595	Add to Cart
BHE10508768	CSB-EL024011RA	5x96T	$2856	Add to Cart
BHE10508768	CSB-EL024011RA	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.