Rat Troponin I - Fast Skeletal Muscle

Mfr.No.

E0925Ra

Sensitivity

3.17ng/L

Detection Range

7-1500ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

TNNI 2;TNNI2;Troponin I, fast skeletal muscle;Troponin I, fast-twitch isoform

Molecular Weight

21328

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0925Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	213.8	8.29	3.9
Intra-Assay	2	18	443.9	18.38	4.1
Intra-Assay	3	18	557.6	13.3	2.4

Background

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Protocol

These standard curves of E0925Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/L	2.047	2.079	2.038
	2.112
400ng/L	0.955	0.974	0.932
	0.993
200ng/L	0.467	0.435	0.394
	0.403
100ng/L	0.161	0.192	0.151
	0.223
50ng/L	0.105	0.136	0.094
	0.167
0ng/L	0.057	0.041	0
	0.026

Gene ID	29389
UniProt ID	P27768

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112176	E0925Ra	48T	$320		Add to Cart
BHE12112176	E0925Ra	96T	$458		Add to Cart

Rat Troponin I - Fast Skeletal Muscle - TNNI2 ELISA Kit

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