Rat triggering receptor expressed on myeloid cells 2(TREM2) ELISA kit

Rat triggering receptor expressed on myeloid cells 2(TREM2) ELISA kit

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Mfr.No.

CSB-EL024405RA

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

triggering receptor expressed on myeloid cells 2, TREM-2, Trem2a, Trem2b, Trem2c, triggering receptor expressed on monocytes 2, triggering receptor expressed on myeloid cells 2a

Recovery

The recovery of human GFAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-104
EDTA plasma (n=4)	93	87-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GFAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	100
	Range %	97-103
1:2	Average %	101
	Range %	98-104
1:4	Average %	95
	Range %	90-100
1:8	Average %	92
	Range %	89-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.437	2.578	2.508	2.412
20	2.241	2.256	2.249	2.153
10	1.778	1.790	1.784	1.688
5	1.205	1.224	1.215	1.119
2.5	0.712	0.738	0.725	0.629
1.25	0.504	0.529	0.517	0.421
0.625	0.248	0.267	0.258	0.162
0	0.095	0.097	0.096	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	TREM2

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Ameliorative effects of glycine in an experimentalnonalcoholic steatohepatitis and its correlationbetween TREM-1 and TREM-2 Zhang-Feng Dou.et al,Am J Transl Res,2016 8(2):284-297

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BHE10508760	CSB-EL024405RA	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.