Rat triggering receptor expressed on myeloid cells 2(TREM2) ELISA kit

Rat triggering receptor expressed on myeloid cells 2(TREM2) ELISA kit

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Mfr.No.

CSB-EL024405RA

Alternative Names

triggering receptor expressed on myeloid cells 2, TREM-2, Trem2a, Trem2b, Trem2c, triggering receptor expressed on monocytes 2, triggering receptor expressed on myeloid cells 2a

Reactivity

Rat

Detection Range

15.6 pg/mL-1000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human GFAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-104
EDTA plasma (n=4)	93	87-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GFAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	100
	Range %	97-103
1:2	Average %	101
	Range %	98-104
1:4	Average %	95
	Range %	90-100
1:8	Average %	92
	Range %	89-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.437	2.578	2.508	2.412
20	2.241	2.256	2.249	2.153
10	1.778	1.790	1.784	1.688
5	1.205	1.224	1.215	1.119
2.5	0.712	0.738	0.725	0.629
1.25	0.504	0.529	0.517	0.421
0.625	0.248	0.267	0.258	0.162
0	0.095	0.097	0.096	?

Species

Rattus norvegicus

Sensitivity

3.9 pg/mL

Limit Of Detection

450 nm

Antigen	Triggering Receptor Expressed On Myeloid Cells 2

Applications

ELISA

Research Area

TREM2

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Ameliorative effects of glycine in an experimentalnonalcoholic steatohepatitis and its correlationbetween TREM-1 and TREM-2 Zhang-Feng Dou.et al,Am J Transl Res,2016 8(2):284-297

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SKU	Supplier No.	Size	Your price
BHE10508760	CSB-EL024405RA	96T	$695	Add to Cart
BHE10508760	CSB-EL024405RA	5x96T	$3336	Add to Cart
BHE10508760	CSB-EL024405RA	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.