Matrices listed below were spiked with certain level of TRACP-5b and the recovery rates were calculated by comparing the measured value to the expected amount of TRACP-5b in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-102
98
EDTA plasma(n=5)
85-104
94
heparin plasma(n=5)
89-104
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TRACP-5b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
TRACP-5b ELISA Kit allows for the in vitro quantitative determination of TRACP-5b concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat TRACP-5b(TartRate-Resistant Acid Phosphatase 5b) ELISA Kit