Rat TPH2(Tryptophan Hydroxylase 2) ELISA Kit

Mfr.No.

ELK6757

Alternative Names

NTPH; Neuronal tryptophan hydroxylase; Tryptophan 5-monooxygenase 2

Reactivity

Rat

Detection Range

1.57-100 ng/mL

Sample Type

Tissue homogenates, Cell lysates, Other biological fluids

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant TPH2 and the recovery rates were calculated by comparing the measured value to the expected amount of TPH2 in samples.

Matrix	Recovery range	Average
serum(n=5)	84-96%	90%
EDTA plasma(n=5)	96-107%	101%
Heparin plasma(n=5)	87-99%	92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TPH2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	93-102%	93-106%	87-101%	91-104%
EDTA plasma(n=5)	88-100%	95-103%	89-97%	85-94%
Heparin plasma(n=5)	87-103%	97-105%	79-90%	87-96%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
100.00	2.013	1.924
50.00	1.709	1.620
25.00	1.121	1.032
12.50	0.895	0.806
6.25	0.539	0.450
3.13	0.351	0.262
1.57	0.216	0.127
0.00	0.089	0.000

Sensitivity

0.56 ng/mL

Limit Of Detection

100 ng/mL

UniProt ID	Q8CGU9
Antigen	TPH2
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TPH2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TPH2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TPH2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TPH2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Enzyme & Kinase;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15204988	ELK6757	48T	$320		Add to Cart
BHE15204988	ELK6757	96T	$458		Add to Cart

Rat TPH2(Tryptophan Hydroxylase 2) ELISA Kit

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