Matrices listed below were spiked with certain level of total SOD and the recovery rates were calculated by comparing the measured value to the expected amount of total SOD in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-102
92
EDTA plasma(n=5)
87-97
92
heparin plasma(n=5)
87-99
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of total SOD and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-101%
87-105%
94-104%
EDTA plasma(n=5)
89-96%
82-99%
82-100%
heparin plasma(n=5)
82-94%
85-96%
80-95%
Sensitivity
< 0.469ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
total SOD ELISA Kit allows for the in vitro quantitative determination of total SOD concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat total SOD(superoxide dismutase) ELISA Kit