Rat Tissue-type Plasminogen Activator

Mfr.No.

E1758Ra

Sensitivity

0.04ng/ml

Detection Range

0.1-40ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

PLAT;Tissue-type plasminogen activator;Tissue-type plasminogen activator chain A;Tissue-type plasminogen activator chain B;tPA;t-PA;t-plasminogen activator

Molecular Weight

62903

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1758Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	7.5	0.41	5.5
Intra-Assay	2	18	14	0.49	3.5
Intra-Assay	3	18	2.2	0.13	5.9

Background

Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. During oocyte activation, plays a role in cortical granule reaction in the zona reaction, which contributes to the block to polyspermy (PubMed: 1515147).

Protocol

These standard curves of E1758Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	2.399	2.533	2.476
	2.667
12ng/ml	1.576	1.493	1.436
	1.411
6ng/ml	0.912	0.876	0.818
	0.839
3ng/ml	0.496	0.495	0.437
	0.494
1.5ng/ml	0.207	0.218	0.16
	0.229
0ng/ml	0.054	0.057	0
	0.06

Gene ID	25692
UniProt ID	P19637

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112964	E1758Ra	48T	$320		Add to Cart
BHE12112964	E1758Ra	96T	$458		Add to Cart

Rat Tissue-type Plasminogen Activator - TPA ELISA Kit

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