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Home / ELISA Kit

Rat Tissue Polypeptide Antigen (TPA) ELISA Kit

SKU : BHE13700195

The new proliferation marker, tissue polypeptide-specific antigen (TPS), representing the specific epitope M3 of tissue polypeptide antigen.

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Abbkine Scientific Co., Ltd.

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Mfr.No.	KTE100043
Reactivity	Rat
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Rat Tissue Polypeptide Antigen (TPA) ELISA Kit employs a two-site sandwich ELISA to quantitate TPA.
Alternative Names	TPA
Detection Method	Colorimetric
Kit Components	Rat Tissue Polypeptide Antigen microplate Rat Tissue Polypeptide Antigen standard Rat Tissue Polypeptide Antigen detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Rat Tissue Polypeptide Antigen (TPA) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TPA. No significant cross-reactivity or interference between Rat TPA and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The new proliferation marker, tissue polypeptide-specific antigen (TPS), representing the specific epitope M3 of tissue polypeptide antigen.
Notes	This Rat Tissue Polypeptide Antigen (TPA) ELISA Kit employs a two-site sandwich ELISA to quantitate TPA in samples. An antibody specific for TPA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTPA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Rat Tissue Polypeptide Antigen (TPA) ELISA Kit employs a two-site sandwich ELISA to quantitate TPA in samples. An antibody specific for TPA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTPA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13700195	KTE100043	48 T	$359		Add to Cart
BHE13700195	KTE100043	96 T	$619		Add to Cart
BHE13700195	KTE100043	96 Tx5	$2809		Add to Cart
BHE13700195	KTE100043	96 Tx50	$26209		Add to Cart

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