Rat Tissue Inhibitors of Metalloproteinase 2

Mfr.No.

E0323Ra

Sensitivity

0.25ng/ml

Detection Range

0.5-90ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Tissue Inhibitors of Metalloproteinase 2 (also known as TIMP-2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Metalloproteinase inhibitor 2;TIMP 2;TIMP2;TIMP-2;Tissue inhibitor of metalloproteinases 2

Molecular Weight

24356

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0323Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	25.3	0.91	3.6
Intra-Assay	2	18	19.8	1.08	5.5
Intra-Assay	3	18	11.8	0.51	4.3

Background

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.

Protocol

These standard curves of E0323Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/ml	2.107	2.191	2.163
	2.275
24ng/ml	1.415	1.384	1.356
	1.353
12ng/ml	0.682	0.673	0.645
	0.664
6ng/ml	0.359	0.39	0.362
	0.421
3ng/ml	0.143	0.173	0.145
	0.204
0ng/ml	0.029	0.028	0
	0.027

Gene ID	29543
UniProt ID	P30121

Research Area	Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111622	E0323Ra	48T	$320		Add to Cart
BHE12111622	E0323Ra	96T	$458		Add to Cart

Rat Tissue Inhibitors of Metalloproteinase 2 - TIMP-2 ELISA Kit

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