Rat tissue inhibitors of metalloproteinase 1,TIMP-1 ELISA Kit

Rat tissue inhibitors of metalloproteinase 1,TIMP-1 ELISA Kit

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Mfr.No.

CSB-E08005r

Sensitivity

0.224 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

TIMP metallopeptidase inhibitor 1, RP1-230G1.3, CLGI, EPA, EPO, FLJ90373, HCI, TIMP, erythroid potentiating activity, fibroblast collagenase inhibitor, tissue inhibitor of metalloproteinase 1

Recovery

The recovery of pig NT-proBNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	105	99-109
EDTA plasma (n=4)	97	90-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig NT-proBNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	103
	Range %	96-108
1:2	Average %	99
	Range %	91-102
1:4	Average %	94
	Range %	86-98
1:8	Average %	98
	Range %	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.129	2.045	2.087	1.949
250	1.671	1.555	1.613	1.475
125	1.134	1.117	1.126	0.988
62.5	0.666	0.650	0.658	0.520
31.2	0.388	0.371	0.380	0.242
15.6	0.257	0.266	0.262	0.124
7.8	0.171	0.167	0.169	0.031
0	0.137	0.139	0.138

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P30120

Applications	ELISA
Research Area	TIMP1

Storage	Store at 2-8°C. Please refer to protocol.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.