Rat tight junction protein 1 (ZO1) ELISA Kit

Rat tight junction protein 1 (ZO1) ELISA Kit

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Mfr.No.

CSB-E17287r

Sensitivity

7.8 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

tight junction protein 1 (ZO1), DKFZp686M05161, MGC133289, ZO-1, tight junction protein 1, tight junction protein ZO-1, zona occludens 1, zonula occludens 1 protein

Recovery

The recovery of human Histatin 5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	97-103
EDTA plasma (n=4)	95	91-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human Histatin 5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-95
1:2	Average %	94
	Range %	90-98
1:4	Average %	100
	Range %	95-104
1:8	Average %	102
	Range %	96-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
800	2.448	2.454	2.451	2.260
400	2.024	2.167	2.096	1.905
200	1.629	1.678	1.654	1.463
100	1.151	1.169	1.160	0.969
50	0.622	0.634	0.628	0.437
25	0.375	0.391	0.383	0.192
12.5	0.312	0.324	0.318	0.127
0	0.192	0.190	0.191	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	ZO1

Storage	Store at 2-8°C. Please refer to protocol.

Reference	The combination of Puerariae Lobatae Radix and Chuanxiong Rhizoma enhanced the absorption and pharmacokinetics of puerarin by modulating the intestinal barrier and influenced gut microbiota Runzhi Chen.et al,Journal of Functional Foods,May 30

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BHE10508724	CSB-E17287r	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.