Rat thyrotropin-releasing hormone,TRH ELISA KIT

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08040r

Alternative Names

thyrotropin-releasing hormone, MGC125964, MGC125965

Reactivity

Rat

Detection Range

0.5 pg/mL-100 pg/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of pig SP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	89	86-92

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig SP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	83
	Range %	80-86
1:2	Average %	93
	Range %	90-96
1:4	Average %	95
	Range %	91-99
1:8	Average %	97
	Range %	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Sensitivity

0.25 pg/mL

Limit Of Detection

450 nm

UniProt ID	P01150
Antigen	thyrotropin-releasing hormone

Applications

ELISA

Research Area

TRH

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Have you used this product?

SKU	Supplier No.	Size	Your price
BHE10508721	CSB-E08040r	96T	$595	Add to Cart
BHE10508721	CSB-E08040r	5x96T	$2856	Add to Cart
BHE10508721	CSB-E08040r	10x96T	$5652.50	Add to Cart