Rat tartrate-resistant acid phosphatase 5b,TRACP-5b ELISA Kit

Mfr.No.

CSB-E08491r

Sensitivity

0.078 mIU/mL

Detection Range

0.312 mIU/mL-20 mIU/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

tartrate-resistant acid phosphatase 5b,TRACP-5b

Recovery

The recovery of human CD244 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	100-108
EDTA plasma (n=4)	97	93-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CD244 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	91
	Range %	88-96
1:2	Average %	102
	Range %	98-106
1:4	Average %	99
	Range %	95-103
1:8	Average %	89
	Range %	85-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	TRACP-5b

Storage	Store at 2-8°C. Please refer to protocol.

Reference	A role for suppressed bone formation favoring catch-up fat in the pathophysiology of catch-up growth after food restriction Xiangfei Guo et al,European Joual of Nutrition,2011 December; 50(8): 645-655 Advanced Oxidation Protein Products Accelerate Bone Deterioration in Aged Rats Zeng JH et al,Exp Gerontol,2013 Dec 4.pii: S0531-5565(13)00343-4. Effects of rabeprazole on bone metabolic disorders in a gastrectomized rat model. Yamasaki Y.et al,Biomed Rep.,5(1):118-124. Boldine isolated from Litsea cubeba inhibits bone resorption by suppressing the osteoclast differentiation in collagen-induced arthritis Zhao H.et al,Int Immunopharmacol.,Aug 18

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