Rat T(Testosterone) ELISA Kit

Mfr.No.

ER1462

Sensitivity

< 0.188ng/ml

Detection Range

0.313-20ng/ml

Reactivity

Rat

Assay Type

Competitive

Alternative Names

Testosterone ELISA Kit

Detection Method

Coated with Antigen

Recovery

Matrices listed below were spiked with certain level of T and the recovery rates were calculated by comparing the measured value to the expected amount of T in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	86-104	95
EDTA plasma(n=5)	86-104	93
heparin plasma(n=5)	88-104	97

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of T and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	89-99%	87-105%	86-88%
EDTA plasma(n=5)	89-98%	82-100%	89-101%
heparin plasma(n=5)	80-94%	82-99%	80-90%

Sample Type

Other liquid samples, Serum, Cell culture supeatants, Cell lysate, Plasma

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Precision

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Notes

For Research Use Only

Applications	ELISA
Applications Notes	T ELISA Kit allows for the in vitro quantitative determination of T concentrations in serum, plasma, tissue homogenates and other biological fluids.

Storage	2-8 °C for 6 months

Reference	Joual: Endocrinological Investigation January 2017, Volume 40, Issue 1, pp 91–100 Title: Lactational exposure effect of polychlorinated biphenyl on rat Sertoli cell markers and functional regulators in prepuberal and puberal F1 offspring Joual: Inteational Joual of Medical Sciences Title: Establishing an Animal Model of Secondary Osteoporosis by Using a Gonadotropin-releasing Hormone Agonist Joual: Food Science and Biotechnology Title: Ameliorative effects of Lycium chinensis on male sexual dysfunction and testicular oxidative damage in streptozotocininduced diabetic rats

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SKU	Supplier No.	Size	Your price	Quantity
BHE10805765	ER1462	96T	$400		Add to Cart

Rat T (Testosterone) ELISA Kit

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