Rat T-cell-specific Surface Glycoprotein Cd28

Mfr.No.

E2275Ra

Sensitivity

0.145ng/mL

Detection Range

0.3-90ng/mL

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

CD antigen CD28;CD28;CD28RNA;T-cell-specific surface glycoprotein CD28

Molecular Weight

25170

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2275Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4.11	0.24	5.76
Intra-Assay	2	18	27.15	0.77	2.83
Intra-Assay	3	18	15.44	0.81	5.23

Background

Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation.

Protocol

These standard curves of E2275Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/mL	2.003	2.085	2.035
	2.168
24ng/mL	1.295	1.399	1.349
	1.504
12ng/mL	0.96	0.913	0.863
	0.867
6ng/mL	0.416	0.449	0.399
	0.482
3ng/mL	0.224	0.268	0.217
	0.311
0ng/mL	0.049	0.05	0
	0.052

Gene ID	25660
UniProt ID	P31042

Research Area	Immunology; Stem Cells

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113407	E2275Ra	48T	$320		Add to Cart
BHE12113407	E2275Ra	96T	$458		Add to Cart

Rat T-cell-specific Surface Glycoprotein Cd28 - CD28 ELISA Kit

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