Rat Syndecan-2(SDC2) ELISA kit

Rat Syndecan-2(SDC2) ELISA kit

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Mfr.No.

CSB-EL020889RA

Sensitivity

0.78 ng/mL

Detection Range

3.12 ng/mL-200 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

syndecan 2, HSPG, HSPG1, SYND2, cell surface-associated heparan sulfate proteoglycan 1, fibroglycan, heparan sulfate proteoglycan 1, cell surface-associated, heparan sulfate proteoglycan core protein, syndecan prot

Recovery

The recovery of canine testosterone spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-95
EDTA plasma (n=4)	87	82-93

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of canine testosterone in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-97
1:2	Average %	97
	Range %	92-103
1:4	Average %	85
	Range %	81-89
1:8	Average %	89
	Range %	83-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
20	0.232	0.236	0.234
5	0.435	0.436	0.436
1.6	0.611	0.613	0.612
0.4	0.984	0.978	0.981
0.1	1.401	1.403	1.402
0	1.985	1.989	1.987

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P34900

Applications	ELISA
Research Area	SDC2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508683	CSB-EL020889RA	96T	$595	Add to Cart
BHE10508683	CSB-EL020889RA	5x96T	$2856	Add to Cart
BHE10508683	CSB-EL020889RA	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.