Rat superoxide dismutase 1, soluble (SOD1) ELISA kit

Rat superoxide dismutase 1, soluble (SOD1) ELISA kit

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Mfr.No.

CSB-EL022397RA

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

superoxide dismutase 1, soluble, ALS, ALS1, IPOA, SOD, homodimer, Cu/Zn superoxide dismutase, SOD, soluble, indophenoloxidase A, superoxide dismutase, cystolic

Recovery

The recovery of rabbit IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	88-103
EDTA plasma (n=4)	95	85-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	89
	Range %	84-97
1:2000	Average %	91
	Range %	89-96
1:4000	Average %	93
	Range %	87-104
1:8000	Average %	92
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
3000	0.221	0.218	0.220
750	0.351	0.354	0.353
187.5	0.629	0.625	0.627
47	1.464	1.502	1.483
12	2.259	2.235	2.247
0	3.218	3.155	3.187

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P07632

Applications	ELISA
Research Area	SOD1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508675	CSB-EL022397RA	96T	$695	Add to Cart
BHE10508675	CSB-EL022397RA	5x96T	$3336	Add to Cart
BHE10508675	CSB-EL022397RA	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.