Rat Sulfotransferase Family 1E Member 1(SULT1E1) ELISA Kit

Rat Sulfotransferase Family 1E Member 1(SULT1E1) ELISA Kit

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Mfr.No.

CSB-E13472r

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

sulfotransferase family 1E, estrogen-preferring, member 1, EST, EST-1, MGC34459, STE, estrogen sulfotransferase, estrone sulfotransferase, sulfotransferase, estrogen-preferring

Recovery

The recovery of human CEMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	91	84-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CEMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-95
1:2	Average %	101
	Range %	97-105
1:4	Average %	83
	Range %	80-86
1:8	Average %	107
	Range %	103-110

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.658	2.697	2.678	2.528
5	2.312	2.331	2.322	2.172
2.5	1.871	1.892	1.882	1.732
1.25	1.312	1.365	1.339	1.189
0.625	0.788	0.797	0.793	0.643
0.312	0.442	0.472	0.457	0.307
0.156	0.281	0.272	0.277	0.127
0	0.151	0.149	0.150

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P52844

Applications	ELISA
Research Area	SULT1E1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508673	CSB-E13472r	96T	$790	Add to Cart
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BHE10508673	CSB-E13472r	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.