Rat Substance P,SP ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08358r

Sensitivity

1.56 pg/mL

Detection Range

6.25 pg/mL-400 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

Substance P,SP

Recovery

The recovery of human CXCL17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-102
EDTA plasma (n=4)	98	90-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CXCL17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	82-94
1:2	Average %	90
	Range %	84-95
1:4	Average %	96
	Range %	92-100
1:8	Average %	93
	Range %	90-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Sample Type

Serum, Cell culture supeatants, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	SP

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Bone adaptation to mechanical loading in a mouse model of reduced peripheral sensory nerve function Heffner MA.et al,PLoS One,Oct 31 Chai-Qin-Cheng-QiDecoction and Carbachol Improve Intestinal Motility by Regulating Protein Kinase C-Mediated Ca2+ Release in Colonic Smooth Muscle Cells in Rats with Acute Necrotising Pancreatitis. Zhang CL.et al,Evid Based Complement Alteat Med. ,Apr 26

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