Rat Substance P Receptor - SP-R ELISA Kit

Mfr.No.

E0073Ra

Description

This sandwich kit is for the accurate quantitative detection of Rat Substance P Receptor (also known as SP-R) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

NK-1 receptor;NK-1R;SPR;Substance-P receptor;Tachykinin receptor 1;TACR 1;TACR1

Reactivity

Rat

Detection Range

20-4500ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

46366

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.32ng/ml

Background

This is a receptor for the tachykinin neuropeptide substance P. It is probably associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance P ; substance K ; neuromedin-K.

Gene ID	24807
UniProt ID	P14600
Antigen	TACR1

Research Area

Others

Protocol

These standard curves of E0073Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/ml	2.417	2.318	2.288
	2.218
1200ng/ml	1.267	1.353	1.323
	1.439
600ng/ml	0.736	0.714	0.685
	0.692
300ng/ml	0.329	0.377	0.347
	0.424
150ng/ml	0.163	0.142	0.113
	0.121
0ng/ml	0.034	0.029	0
	0.025

Precision

We measured random samples of E0073Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1574.6	45.57	2.9
Intra-Assay	2	18	722.3	31.5	4.4
Intra-Assay	3	18	586.7	31.15	5.3

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111393	E0073Ra	48T	$320		Add to Cart
BHE12111393	E0073Ra	96T	$458		Add to Cart

Rat Substance P Receptor - SP-R ELISA Kit

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