Rat Stromal cell derived factor 1a,SDF-1a ELISA Kit

Rat Stromal cell derived factor 1a,SDF-1a ELISA Kit

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Mfr.No.

CSB-E08729r

Alternative Names

Stromal cell derived factor 1a,SDF-1a

Reactivity

Rat

Detection Range

0.78 pg/mL-50 pg/mL

Sample Type

Serum, Cell culture supernatants, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human RSPO2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	86-95
EDTA plasma (n=4)	95	89-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human RSPO2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-91
1:2	Average %	99
	Range %	94-102
1:4	Average %	93
	Range %	86-95
1:8	Average %	94
	Range %	89-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	1.974	1.913	1.944	1.792
5	1.298	1.277	1.288	1.136
2.5	0.739	0.754	0.747	0.595
1.25	0.426	0.415	0.421	0.269
0.625	0.301	0.287	0.294	0.142
0.312	0.234	0.251	0.243	0.091
0.156	0.198	0.192	0.195	0.043
0	0.154	0.150	0.152

Species

Rattus norvegicus

Sensitivity

0.195 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q9QZD1
Antigen	Stromal cell derived factor 1a,SDF-1a

Applications

ELISA

Research Area

SDF1a, CXCL12

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Auditory stimulation modulates CXCL12/CXCR4 expression in postnatal development of the newborn rat cochlea Zhang W. et al,Neuroreport,2015 Aug 19;26(12):681-7. Omega-3 Fatty Acids Supplementation Differentially Modulates the SDF-1/CXCR-4 Cell Homing Axis in Hypertensive and Normotensive Rats Halmenschlager L.et al,Nutrients.,Aug 1

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SKU	Supplier No.	Size	Your price
BHE10508668	CSB-E08729r	96T	$615	Add to Cart
BHE10508668	CSB-E08729r	5x96T	$2952	Add to Cart
BHE10508668	CSB-E08729r	10x96T	$5842.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.