Rat Stromal cell derived factor 1a,SDF-1a ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08729r

Sensitivity

0.195 pg/mL

Detection Range

0.78 pg/mL-50 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Stromal cell derived factor 1a,SDF-1a

Recovery

The recovery of human RSPO2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	86-95
EDTA plasma (n=4)	95	89-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human RSPO2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-91
1:2	Average %	99
	Range %	94-102
1:4	Average %	93
	Range %	86-95
1:8	Average %	94
	Range %	89-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q9QZD1

Applications	ELISA
Research Area	SDF1a, CXCL12

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Auditory stimulation modulates CXCL12/CXCR4 expression in postnatal development of the newborn rat cochlea Zhang W. et al,Neuroreport,2015 Aug 19;26(12):681-7. Omega-3 Fatty Acids Supplementation Differentially Modulates the SDF-1/CXCR-4 Cell Homing Axis in Hypertensive and Normotensive Rats Halmenschlager L.et al,Nutrients.,Aug 1

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