Rat Steroid 5-alpha-reductase 2

Mfr.No.

E1585Ra

Sensitivity

0.024ng/ml

Detection Range

0.05-30ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Steroid 5-Alpha-Reductase 2 (also known as SRD5A2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

3-oxo-5-alpha-steroid 4-dehydrogenase 1;S5AR 1;SR type 1;SRD5A1;Steroid 5-alpha-reductase 1

Molecular Weight

29780

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1585Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2.3	0.14	6
Intra-Assay	2	18	4.9	0.33	6.8
Intra-Assay	3	18	7.2	0.27	3.7

Background

Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.

Protocol

These standard curves of E1585Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
16ng/ml	2.195	2.251	2.203
	2.307
8ng/ml	1.439	1.48	1.432
	1.522
4ng/ml	0.732	0.822	0.774
	0.912
2ng/ml	0.414	0.438	0.39
	0.461
1ng/ml	0.278	0.283	0.235
	0.288
0ng/ml	0.045	0.048	0
	0.052

Gene ID	24950
UniProt ID	P24008

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112804	E1585Ra	48T	$320		Add to Cart
BHE12112804	E1585Ra	96T	$458		Add to Cart

Rat Steroid 5-alpha-reductase 2 - SRD5A2 ELISA Kit

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