Rat Steroid 5-alpha-reductase 1

Mfr.No.

E1584Ra

Sensitivity

3.26ng/L

Detection Range

6-1800ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Steroid 5-Alpha-Reductase 1 (also known as SRD5A1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

3-oxo-5-alpha-steroid 4-dehydrogenase 1;S5AR 1;SR type 1;SRD5A1;Steroid 5-alpha-reductase 1

Molecular Weight

29780

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1584Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	416.6	24.79	6
Intra-Assay	2	18	447.3	10.52	2.4
Intra-Assay	3	18	188.1	13.2	7

Background

Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.

Protocol

These standard curves of E1584Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
960ng/L	2.412	2.425	2.37
	2.438
480ng/L	1.342	1.354	1.299
	1.366
240ng/L	0.833	0.781	0.727
	0.73
120ng/L	0.432	0.431	0.376
	0.429
60ng/L	0.313	0.306	0.251
	0.299
0ng/L	0.067	0.055	0
	0.042

Gene ID	24950
UniProt ID	P24008

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112803	E1584Ra	48T	$320		Add to Cart
BHE12112803	E1584Ra	96T	$458		Add to Cart

Rat Steroid 5-alpha-reductase 1 - SRD5A1 ELISA Kit

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