Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of Sp1 and the recovery rates were calculated by comparing the measured value to the expected amount of Sp1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-104
95
EDTA plasma(n=5)
85-102
93
heparin plasma(n=5)
89-105
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Sp1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sp1 ELISA Kit allows for the in vitro quantitative determination of Sp1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat Sp1 (Transcription factor Sp1) ELISA Kit